S1 nuclease protection mapping

S1 Mapping is a laboratory method used for locating the start and end points of transcripts and for mapping introns.

This protocol provides details for nuclease S1 mapping of mRNA using a uniformly labeled, single-stranded DNA probe. DNA-RNA hybrids are ...

The basic S1 nuclease mapping technique consists of hybridizing a labeled DNA probe that partially overlaps a RNA species of interest to that RNA species and ...

Nuclease-$1 mapping is used to map the locations of the ends of RNA mole- cules and of any splice points within them in relation to specific sites (e.g.,.

S1 nuclease mapping is mainly used to map transcription start sites accurately (to be described in this chapter), this method can also be used to map intron- ...

S1 Mapping. Nuclease S1 will digest only ssDNA or ssRNA. If a duplex of DNA and/or RNA strands have single-stranded overhangs or unhybridized ...

S1 nuclease mapping is a laboratory technique used to locate the 5' end of an RNA transcript within a mixture by using the S1 nuclease.

The method can be used to quantify RNAs, to map the positions of introns and to identify the locations of 5′ and 3′ ends of mRNAs on cloned DNA templates.

The first step in mapping the transcriptional start site by S1 nuclease treatment is to clone the upstream region of the gene into an M13 vector. Next, single- ...